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Biotin-16-UTP 10 mM Solution
- Commodity name： Biotin-16-UTP 10 mM Solution
- 货号： OLV1503
- Product Description
-
Technical Parameters
Purity
99%
Storage conditions
-20 ± 5℃
pH
8.0
Molecular formula
C 32 H 48 N 7 No 4 O 19 P 3 S
Molecular weight
987.5
Specifications
1ml
Product abbreviation
Biotin-16-UTP
Chinese name
Biotin-16-UTP
Product Description
Biotin-16-UTP, as an active substrate for RNA polymerase, can replace UTP in in vitro transcription reactions to label RNA.
I. Basic Information
Chinese name: Biotin-16-UTP
English name: Biotin-16-UTP
CAS: 186033-13-6
II. Structural Features
Biotin: a small-molecule vitamin that can specifically bind to avidin or streptavidin.
16-carbon chain (e.g., 16-aminocaproic acid linker): a flexible long chain used to connect biotin and UTP, which helps reduce steric hindrance and enhance binding efficiency.
Uridine triphosphate (UTP): a nucleotide triphosphate that serves as an essential precursor in the process of RNA synthesis.
III. Application Fields
In vitro transcription using UTP substitutes: It can serve as a substrate for SP6, T3, and T7 RNA polymerases, replacing UTP in in vitro transcription reactions to generate biotin-labeled RNA.
Hybridization probes: These labeled RNAs can serve as hybridization probes and are useful in experiments such as Northern blotting, Southern blotting, dot or colony blots, RNase protection assays, in situ hybridization, and microarray hybridization.
RNA-protein interactions: Biotin-labeled RNA can be used to study interactions between RNA and proteins, such as the identification and functional analysis of RNA-binding proteins.
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