Termination ddNTP Kit (for MALDI-TOF MS, 10 mM)

Commodity name： Termination ddNTP Kit (for MALDI-TOF MS, 10 mM)
货号： OLV3007

Category:

Protected Nucleosides

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wangtongtong@cnorilife.com

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+86-15032236037

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Product Description

Mastering the ddNTP modification technology for nucleic acid mass spectrometry,

Termination-ddNTP (for MALDI-TOF MS) is now officially available!

Nucleic acid mass spectrometry is a multiplex PCR-based detection system that has evolved from MALDI-TOF MS mass spectrometry. In recent years, it has emerged as a new molecular diagnostic platform following PCR and NGS. While fluorescence quantitative PCR offers rapid detection but has limited throughput and cannot easily and quickly meet the demand for analyzing dozens to hundreds of sites across multiple genes, high-throughput sequencing, though boasting extremely high throughput, comes with high testing costs, long project turnaround times, and requires specialized analysis and interpretation of the generated data—thus presenting a relatively high technical barrier. In contrast, nucleic acid mass spectrometry delivers stable and accurate results at a lower cost, perfectly addressing the need for qualitative and quantitative detection of SNPs and gene mutations at moderate-throughput loci. Moreover, it enables convenient and rapid detection of DNA methylation and copy number variations (CNVs), further highlighting its strong competitiveness in the field of genetic testing and making it one of the rapidly developing mainstays in life sciences, particularly in clinical diagnostics.

Nucleic acid mass spectrometry primarily relies on multiplex PCR, high-throughput chips, and time-of-flight mass spectrometry to perform nucleic acid detection. This technology enables the simultaneous detection of dozens or even hundreds of target markers in a single sample, with the capacity to process over 1,000 samples per day.

The main steps of nucleic acid mass spectrometry are as follows:

1. Polymerase Chain Reaction (PCR): Design one forward primer and one reverse primer targeting the locus to be detected. Using the sample DNA as a template, perform PCR to obtain a PCR product fragment containing the target locus.

2. Treatment with shrimp alkaline phosphatase (SAP): SAP is added to the PCR product to perform a dephosphorylation reaction, thereby removing any residual dNTPs from the reaction system.

3. Single-base extension reaction: Add a single-base extension primer and the extension termination mix containing ddNTPs to perform the single-base extension reaction.

4. Resin Purification: Add desalting resin to the extended product for desalting and purification, thereby preventing salt ion peaks from interfering with the mass spectrometry results.

5. Sample-matrix binding: The sample is spotted onto a chip coated with a matrix, and then subjected to mass spectrometry analysis. Different gene fragments ultimately generate distinct detection peak profiles.

The core technology underlying nucleic acid mass spectrometry is single-base extension technology, which relies on ddNTPs as chain-terminating agents. By analyzing the extension products generated in the single-base extension reaction, this method achieves the goal of sequencing nucleotide sequences.

Currently, the resolution of nucleic acid mass spectrometers on the market is around 10 Da. During single-base extension, the molecular weights of ordinary ddATP and ddTTP differ by just under 10 Da, making it impossible for the mass spectrometer’s resolution to accurately distinguish between A and T, thus leading to detection failures. This challenge has become one of the key technical barriers limiting the clinical application of nucleic acid mass spectrometry.

To break through this technological barrier, Olivebio has devoted itself to intensive R&D and finally mastered the ddNTP modification technology for nucleic acid mass spectrometry. We are proud to introduce our specialized Termination-ddNTP (for MALDI-TOF MS) product for nucleic acid mass spectrometry! After modification, the molecular weight differences among these ddNTPs (for MALDI-TOF MS) exceed the resolution of mass spectrometers (10 Da), enabling nucleic acid mass spectrometers to precisely distinguish between A, T, C, and G, thereby ensuring the accuracy of detection.

Product Features

1. Dedicated for Nucleic Acid Mass Spectrometry: This product is specifically designed for MALDI-TOF MS. The molecular weight differences between the modified ddNTPs are all greater than 10 Da, ensuring accurate mass spectrometry detection!

2. High purity, HPLC >99%

3. Stability: Good batch-to-batch stability and a rigorous quality management system.

4. Residue-free—no residual DNase or nuclease activity, no residual human or bacterial genomic DNA.